Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Devices’ StakMax® Microplate Stacker in a matter of minutes and . ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. With optimized reagents, option to operate the. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . It also integrates with the. 100 readings per well gives optimal Z’ values for this assay. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. No additional manuals are available. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. Figure 1. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The guide also covers the software features, the specifications, and the accessories of the systems. For SpectraMax ABS Plus reader, r 2 = 0. SAN JOSE, CA — Molecular Devices, LLC. Turn the reader off by pulling the power cord. Figure 1. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. M2. 315 can't find zero order. Example: [Drawer]. The chamber contains springs that position the cuvette in the proper alignment for a read. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. microplates or in a cuvette at a. . Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The dual monochromator optics allow the widest range of. SoftMax® Pro 7. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Time-. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. FI assay. 2 software: see SoftMax® Pro 7. 6% for the on-board optics (monochromators). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. In addition, the iD5 reader measures TRF and FP. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraMax ® M2 Multi-Mode Microplate Reader. SpectraMax Mini User Guide. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Table 1. 100 fmol 1536-well (8 µL) 0. The plate reader optics are tunable for absorbance. Order Now. View the Molecular Devices website. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. 0 out of 5 $ 9,995 $ 9,495. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. Create custom fluorescence assays without having to purchase expensive filter sets. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Use the provided 3. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Instrument settings used with the SpectraMax M5 Microplate Reader. Offer Ends In: 2 Days 10: 40: 19. Results are shown in Table 1. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 40266 Email: drugdiscoverytech@lifetech. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 3) This warranty. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices. flexibility of monochromator-based systems. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The calculated LOQ values were 0. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). limit of detection (LLD) for ATP was estimated for each microplate reader using the . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. In . The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. ELISA Plate Readers / Microplate Readers. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. microplate reader. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Fluorescence Microplate Reader Comparison Chart. Note that this procedure is only for the SpectraMax L model. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. both cuvette-based and microplate reader applications on the same instrument. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Supplier: Molecular Devices. Using the. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Guides: SpectraMax Mini Installation Guide. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Request a Quote!Introduction. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. both cuvette-based and microplate reader applications Microplate Readers. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. To resume collecting kinetic measurements within the same file, press the Append button. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 305 reference level saturation. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. . 1. Temperature control and shaking come as standard. This unlocks the transport slide. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Unlock your i3/i3x instrument after shipment. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Please refer to the Obsolete Microplate Reader Models for more information. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 1. The calculated LOQ values were 0. The temperature in the plate chamber is ambient and isothermal. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 0200-6263. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 1. . Add to Cart. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Path Check Pathlength Measurement SpectraMax i3x. For more sample throughput, both readersSupplier: Molecular Devices. Molecular Devices Spectramax iD3 Microplate Reader. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Step 2: Launch SoftMax Pro Software. 0 out of 5 $ 5,999. . SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. The CellTiter-Glo assay from Promega uses luciferase. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Reader modes are customized to your specific applications with options to upgrade easily. 1) Select the correct plate and the wells that you want to measure. 76358-624. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 5 87. The instrument uses a paired monochromator and xenon flash. 5 12. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Maximum signal with lowest background. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Selection of Water Constant in PathCheck settings. SpectraMax Spectrofluorometers. Our specialization servicing. Wide range of applications. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The following document is intended to demonstrate setup of this instrument. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Introduction. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. 020 fmol 0. On the back, in the right hand top corner,. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Ease of use. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Compact designs. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. A preconfigured protocol is available in the software to. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Cuvette Microplate wells. 305 reference level saturation. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Our absorbance plate readers feature our PathCheck Sensor technology and. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. When the user applies a set temperature of 37°C, the top is heated to 37. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. Contents of wells were pipetted up and down several times to mix. SpectraMax M5 reader settings are shown. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 5 87. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Figure 1. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 2. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In . Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Step 1: Open the SoftMax Pro IMAP FP protocol. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Table of Contents. . SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Also see:. Microplate Readers. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. B: Selection of Excitation Wavelength. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. SpectraMax 250 User. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Fill a clean plate with water. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Users can perform spectral scans from 250 nm to 750. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 997 (n = 3). The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. SpectraDrop High-Throughput Screening (HTS) Kit. SpectraMax Microplate Readers. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Transcreener ® ADP. Table 1. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Additionally, user-installable options allow the SpectraMax i3 System to. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Most papers they cultured bacteria to OD600>0. These robust readers have been placed in labs from. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The dual monochromator optics allow the. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. begin reading microplates with seven mouse clicks. Sale. 20 μL of Component A was added to each well of the microplate. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. \ Figure 3. Path Check Pathlength Measurement every Gemini EM Reader. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. A. com 4. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. We would like to show you a description here but the site won’t allow us. Unparalleled performance on a personalized platform. San Jose, Calif. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Download PDF. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. 2. 6% for the on-board optics (monochromators). The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Devices’ StakMax® Microplate Stacker in a matter of minutes and . degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Highly customizable low volume. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Or scan up to 384 wells at any time to determine the best assay conditions. 306 plate air cal. western blot membrane detection in a multimode microplate reader platform. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Detection modalities include absorbance (UV-Vis Abs) and. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. 72 MB. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The guide also covers the software features, the specifications, and the accessories of the systems. Find our technical support information along with contact details. Other features and benefits include: Eliminate changing out filters. Total volume (except deep. Absorbance (optical density, OD) was read at 570 nm and 605. Cell Analysis. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. With optimized reagents, option to operate the. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.